Quantification of protein carbonylation
نویسندگان
چکیده
منابع مشابه
Biochemical Aspects of Protein Changes in Seed Physiology and Germination
Seed storage proteins are synthesized as sources of carbon, nitrogen and sulfur for the next generation of plants. Reactive oxygen species serve as second messengers for signal transduction; however, molecular targets of oxidant signaling have not been defined. Here, many researchers showes that ligand–receptor mediated signaling promotes reactive oxygen species– dependent protein carbonylation...
متن کاملBiochemical Aspects of Protein Changes in Seed Physiology and Germination
Seed storage proteins are synthesized as sources of carbon, nitrogen and sulfur for the next generation of plants. Reactive oxygen species serve as second messengers for signal transduction; however, molecular targets of oxidant signaling have not been defined. Here, many researchers showes that ligand–receptor mediated signaling promotes reactive oxygen species– dependent protein carbonylation...
متن کاملIn situ visualization of carbonylation and its co-localization with proteins, lipids, DNA and RNA in Caenorhabditis elegans.
All key biological macromolecules are susceptible to carbonylation - an irreparable oxidative damage with deleterious biological consequences. Carbonyls in proteins, lipids and DNA from cell extracts have been used as a biomarker of oxidative stress and aging, but formation of insoluble aggregates by carbonylated proteins precludes quantification. Since carbonylated proteins correlate with and ...
متن کاملDynamics of protein damage in yeast frataxin mutant exposed to oxidative stress.
Oxidative stress and protein carbonylation is implicated in aging and various diseases such as neurodegenerative disorders, diabetes, and cancer. Therefore, the accurate identification and quantification of protein carbonylation may lead to the discovery of new biomarkers. We have developed a new method that combines avidin affinity selection of carbonylated proteins with iTRAQ labeling and LC ...
متن کاملCytoprotective Effects of Hydrophilic and Lipophilic Extracts of Pistacia vera against Oxidative Versus Carbonyl Stress in Rat Hepatocytes
This study was conducted to evaluate the cytoprotection of various extracts and bioactive compounds found in Pistacia vera againts cytotoxicity, ROS formation, lipid peroxidation, protein carbonylation , mitochondrial and lysosomal membrane damages in cell toxicity models of diabetes related carbonyl (glyoxal) and oxidative stress (hydroperoxide). Methanol, water and ethyl acetate were used to ...
متن کامل